Rabbit Polyclonal Antibody to RPL27A

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别名:60S ribosomal protein L27a, RPL27A
应用:WB,IHC,FCM
反应种属:Human, Hamster
规格：50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.

Description

Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L15P family of ribosomal proteins. It is located in the
cytoplasm. Variable expression of this gene in colorectal cancers
compared to adjacent normal tissues has been observed, although no
correlation between the level of expression and the severity of the
disease has been found. As is typical for genes encoding ribosomal
proteins, multiple processed pseudogenes derived from this gene are
dispersed through the genome.

Specification
Aliases	60S ribosomal protein L27a, RPL27A
Entrez GeneID	6157
Swissprot	P46776
WB Predicted band size	16.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Hamster
Immunogen	This RPL27A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 110-138 amino acids from the C-terminal region of human RPL27A.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	RPL27A Antibody (C-term) (Cat. #P30877) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
	RPL27A Antibody (C-term) (Cat. #P30877) western blot analysis in CHO cell line lysates (35ug/lane).This demonstrates the RPL27A antibody detected the RPL27A protein (arrow).
	RPL27A Antibody (C-term) (Cat. #P30877) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	RPL27A Antibody (C-term) (Cat. #P30877) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPL27A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.