Rabbit Polyclonal Antibody to RPL3

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别名:60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B, RPL3
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene’s introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq].

Description

Ribosomes, the complexes that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 60S subunit. The protein belongs
to the L3P family of ribosomal proteins and it is located in the
cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has
been suggested that the protein contributes to tat-mediated
transactivation. This gene is co-transcribed with several small
nucleolar RNA genes, which are located in several of this gene’s
introns. Alternate transcriptional splice variants, encoding
different isoforms, have been characterized. As is typical for
genes encoding ribosomal proteins, there are multiple processed
pseudogenes of this gene dispersed through the genome. [provided by
RefSeq].

Specification
Aliases	60S ribosomal protein L3, HIV-1 TAR RNA-binding protein B, TARBP-B, RPL3
Entrez GeneID	6122
Swissprot	P39023
WB Predicted band size	46.1kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This RPL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224-251 amino acids from the Central region of human RPL3.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	RPL3 Antibody (Center) (Cat. #P31281) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the RPL3 antibody detected the RPL3 protein (arrow).
	RPL3 Antibody (Center) (Cat. #P31281)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of RPL3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.