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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to RPS2 (N-Term)

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别名:40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4
应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Specification
Aliases 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4
Entrez GeneID 6187
Swissprot P15880
WB Predicted band size 31.3kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This RPS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 45-79 amino acids from human RPS2.
Application
WB 1/2000
FCM 1/25

All lanes : Anti-RPS2 Antibody (N-Term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: NIH/3T3 whole cell lysate
Lane 3: Jurkat whole cell lysate
Lane 4: Hela whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 31 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained with P34363(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34363, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。