别名:40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4应用:WB,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Specification | |
---|---|
Aliases | 40S ribosomal protein S2, 40S ribosomal protein S4, Protein LLRep3, RPS2, RPS4 |
Entrez GeneID | 6187 |
Swissprot | P15880 |
WB Predicted band size | 31.3kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This RPS2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 45-79 amino acids from human RPS2. |
Application | |
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WB | 1/2000 |
FCM | 1/25 |
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All lanes : Anti-RPS2 Antibody (N-Term) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: NIH/3T3 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing Jurkat cells stained with P34363(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34363, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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