Rabbit Polyclonal Antibody to RPS3A

包装: 1G|100G|500G|1KG|100KG

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别名:40S ribosomal protein S3a {ECO:0000255|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255|HAMAP-Rule:MF_03122}, FTE1, MFTL
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. Transcript variants utilizing alternative transcription start sites have been described. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Description

Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a ribosomal
protein that is a component of the 40S subunit. The protein belongs
to the S3AE family of ribosomal proteins. It is located in the
cytoplasm. Disruption of the gene encoding rat ribosomal protein
S3a, also named v-fos transformation effector protein, in
v-fos-transformed rat cells results in reversion of the transformed
phenotype. Transcript variants utilizing alternative transcription
start sites have been described. This gene is co-transcribed with
the U73A and U73B small nucleolar RNA genes, which are located in
its fourth and third introns, respectively. As is typical for genes
encoding ribosomal proteins, there are multiple processed
pseudogenes of this gene dispersed through the genome.

Specification
Aliases	40S ribosomal protein S3a {ECO:0000255\|HAMAP-Rule:MF_03122}, v-fos transformation effector protein, Fte-1, RPS3A {ECO:0000255\|HAMAP-Rule:MF_03122}, FTE1, MFTL
Entrez GeneID	6189
Swissprot	P61247
WB Predicted band size	29.9kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This RPS3A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 228-254 amino acids from the C-terminal region of human RPS3A.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	RPS3A Antibody (C-term) (Cat. #P30697) western blot analysis in Hela,A2058 cell line lysates (15ug/lane).This demonstrates the RPS3A antibody detected the RPS3A protein (arrow).
	RPS3A Antibody (C-term) (Cat. #P30697) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	RPS3A Antibody (C-term) (Cat. #P30697) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS3A Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.