Rabbit Polyclonal Antibody to RPS6 (Ser240/244)

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别名:40S ribosomal protein S6, Phosphoprotein NP33, RPS6
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Description

Ribosomes, the organelles that catalyze protein synthesis,
consist of a small 40S subunit and a large 60S subunit. Together
these subunits are composed of 4 RNA species and approximately 80
structurally distinct proteins. This gene encodes a cytoplasmic
ribosomal protein that is a component of the 40S subunit. The
protein belongs to the S6E family of ribosomal proteins. It is the
major substrate of protein kinases in the ribosome, with subsets of
five C-terminal serine residues phosphorylated by different protein
kinases. Phosphorylation is induced by a wide range of stimuli,
including growth factors, tumor-promoting agents, and mitogens.
Dephosphorylation occurs at growth arrest. The protein may
contribute to the control of cell growth and proliferation through
the selective translation of particular classes of mRNA. As is
typical for genes encoding ribosomal proteins, there are multiple
processed pseudogenes of this gene dispersed through the genome.

Specification
Aliases	40S ribosomal protein S6, Phosphoprotein NP33, RPS6
Entrez GeneID	6194
Swissprot	P62753
WB Predicted band size	28.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This RPS6 (Ser240/244) Antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-249 amino acids from human RPS6.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	Western blot analysis of lysate from HeLa cell line, using RPS6 Antibody(Ser240/244)(Cat. #P30754). P30754 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	RPS6 Antibody (Ser240/244) (Cat. #P30754) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	RPS6 antibody (Ser240/244) (Cat. #P30754) immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RPS6 antibody (Ser240/244) for immunohistochemistry. Clinical relevance has not been evaluated.