别名:Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl
Description |
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RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways. |
Specification | |
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Aliases | Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903) |
Entrez GeneID | 58528 |
Swissprot | Q9NQL2 |
WB Predicted band size | 45.6kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse |
Immunogen | This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/1000 |
IHC | 1/100-1/500 |
FCM | 1/10-1/50 |
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Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 46 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of RRAGD Antibody (Center) (Cat. #P34193) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab. |
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Western blot analysis of RRAGD Antibody (Center) (Cat. #P34193) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab. |
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RRAGD Antibody (Center) (Cat. #P34193) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
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RRAGD Antibody (Center) (Cat. #P34193) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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