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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to RRAGD

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别名:Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)
应用:WB,IHC,FCM
反应种属:Human, Mouse
规格:50μl/100μl

Description
RRAGD is a monomeric guanine nucleotide-binding protein, or G protein. By binding GTP or GDP, small G proteins act as molecular switches in numerous cell processes and signaling pathways.
Specification
Aliases Ras-related GTP-binding protein D, Rag D, RagD, RRAGD (HGNC:19903)
Entrez GeneID 58528
Swissprot Q9NQL2
WB Predicted band size 45.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This RRAGD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-199 amino acids from the Central region of human RRAGD.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
IHC 1/100-1/500
FCM 1/10-1/50

Anti-RRAGD Antibody (Center) at 1:1000 dilution + 293T/17 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 46 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Western blot analysis of RRAGD Antibody (Center) (Cat. #P34193) in MDA-MB435 cell line lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.
Western blot analysis of RRAGD Antibody (Center) (Cat. #P34193) in mouse cerebellum tissue lysates (35ug/lane). RRAGD (arrow) was detected using the purified Pab.
RRAGD Antibody (Center) (Cat. #P34193) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
RRAGD Antibody (Center) (Cat. #P34193) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the RRAGD Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。