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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to RSBN1 (N-term)

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别名:Round spermatid basic protein 1, RSBN1
应用:WB,IHC
反应种属:Human, Mouse
规格:50μl/100μl

Specification
Aliases Round spermatid basic protein 1, RSBN1
Entrez GeneID 54665
Swissprot Q5VWQ0
WB Predicted band size 90.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This RSBN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 51-78 amino acids from the N-terminal region of human RSBN1.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
IHC 1/100-1/500

All lanes : Anti-RSBN1 Antibody (N-term) at 1:2000 dilution
Lane 1: CCRF-CEM whole cell lysate
Lane 2: Jurkat whole cell lysate
Lane 3: human liver lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: HL-60 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 90 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33462 staining RSBN1 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。