Rabbit Polyclonal Antibody to RT34 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:28S ribosomal protein S34, mitochondrial, MRP-S34, S34mt, MRPS34
应用:WB,FCM
反应种属:Human, Mouse
规格：50μl/100μl

Description
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. Alternate splice variants of this gene have been described but their full-length nature has not been determined.

Description

Mammalian mitochondrial ribosomal proteins are encoded by
nuclear genes and help in protein synthesis within the
mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a
small 28S subunit and a large 39S subunit. They have an estimated
75% protein to rRNA composition compared to prokaryotic ribosomes,
where this ratio is reversed. Another difference between mammalian
mitoribosomes and prokaryotic ribosomes is that the latter contain
a 5S rRNA. Among different species, the proteins comprising the
mitoribosome differ greatly in sequence, and sometimes in
biochemical properties, which prevents easy recognition by sequence
homology. This gene encodes a 28S subunit protein. Alternate splice
variants of this gene have been described but their full-length
nature has not been determined.

Specification
Aliases	28S ribosomal protein S34, mitochondrial, MRP-S34, S34mt, MRPS34
Entrez GeneID	65993
Swissprot	P82930
WB Predicted band size	25.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This RT34 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human RT34.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
FCM	1/10-1/50

	RT34 Antibody (N-term) (Cat. #P30887) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the RT34 antibody detected the RT34 protein (arrow).
	RT34 Antibody (N-term) (Cat. #P30887) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.