Rabbit Polyclonal Antibody to SCEL

包装: 1G|100G|500G|1KG|100KG

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别名:Sciellin, SCEL
应用:WB,IHC,FCM
反应种属:Human
规格：50μl/100μl

Description
The protein encoded by this gene is a precursor to the cornified envelope of terminally differentiated keratinocytes. This protein localizes to the periphery of cells and may function in the assembly or regulation of proteins in the cornified envelope. Transcript variants encoding different isoforms exist. A transcript variant utilizing an alternative polyA signal has been described in the literature, but its full-length nature has not been determined.

Description

The protein encoded by this gene is a precursor to the
cornified envelope of terminally differentiated keratinocytes. This
protein localizes to the periphery of cells and may function in the
assembly or regulation of proteins in the cornified envelope.
Transcript variants encoding different isoforms exist. A transcript
variant utilizing an alternative polyA signal has been described in
the literature, but its full-length nature has not been determined.

Specification
Aliases	Sciellin, SCEL
Entrez GeneID	8796
Swissprot	O95171
WB Predicted band size	77.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This SCEL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 260-289 amino acids from the Central region of human SCEL.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/500
FCM	1/25

	Anti-SCEL Antibody (Center) at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Overlay histogram showing A431 cells stained with P34599(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
	Immunohistochemical analysis of paraffin-embedded human skin tissue using P34599 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
	Immunohistochemical analysis of paraffin-embedded human esophagus tissue using P34599 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.