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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to SCF (KITLG)

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别名:Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF
应用:WB,ICC,FCM
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
KITLG is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. This ligand is a pleiotropic factor that acts in utero in germ cell and neural cell development, and hematopoiesis, all believed to reflect a role in cell migration. In adults, it functions pleiotropically, while mostly noted for its continued requirement in hematopoiesis.
Specification
Aliases Kit ligand, Mast cell growth factor, MGF, Stem cell factor, SCF, c-Kit ligand, Soluble KIT ligand, sKITLG, KITLG, MGF, SCF
Entrez GeneID 4254
Swissprot P21583
WB Predicted band size 30.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG).
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Application
WB 1/1000
ICC 1/10-1/50
FCM 1/10-1/50

Western blot analysis of KITLG Antibody (C-term) (Cat.#P30216) in 293 cell line lysates (35ug/lane). KITLG (arrow) was detected using the purified Pab.
Western blot analysis of KITLG(arrow) using rabbit polyclonal KITLG Antibody (C-term) (Cat.#P30216). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2) (Origene Technologies).
Immunofluorescence analysis of anti-KITLG Antibody (C-term) (Cat.#P30216) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。