中文 
别名:Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1应用:WB,IHC,ICC
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Specification | |
|---|---|
| Aliases | Signal peptide, CUB and EGF-like domain-containing protein 2, Protein CEGP1, SCUBE2, CEGP1 |
| Entrez GeneID | 57758 |
| Swissprot | Q9NQ36 |
| WB Predicted band size | 110.0kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SCUBE2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 251-280 amino acids from the Central region of human SCUBE2. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| ICC | 1/10-1/50 |
 |
All lanes : Anti-SCUBE2 Antibody (Center N266) at 1:1000 dilution Lane 1: Hela whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size : 90kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Western blot analysis of lysates from Hela, ZR-75-1, HT-29 cell line (from left to right), using SCUBE2 Antibody (Center N266)(Cat. #P34924). P34924 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane. |
 |
SCUBE2 Antibody (Center N266) (Cat. #P34924) western blot analysis in MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).This demonstrates the SCUBE2 antibody detected the SCUBE2 protein (arrow). |
 |
Confocal immunofluorescent analysis of SCUBE2 Antibody (Center N266)(Cat#P34924) with ZR-75-1 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue). |
 |
SCUBE2 Antibody (Center N266) (Cat. #P34924)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCUBE2 Antibody (Center N266) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
暂无评论
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
发表回复