库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to SEC24B

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  • 满200元免运费
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别名:Protein transport protein Sec24B, SEC24-related protein B, SEC24B
应用:WB,FCM
反应种属:Human
规格:50μl/100μl

Description
Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
Specification
Aliases Protein transport protein Sec24B, SEC24-related protein B, SEC24B
Entrez GeneID 10427
Swissprot O95487
WB Predicted band size 137.4kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This SEC24B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1209-1239 amino acids from the human region of human SEC24B.
Application
WB 1/2000
FCM 1/25

Anti-SEC24B Antibody (C-Term) at 1:2000 dilution + 293 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 137 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing A431 cells stained with P34509(green line). The cells were fixed with 2% paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at Room temperature. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。