Rabbit Polyclonal Antibody to SERPINI1 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:Neuroserpin, Peptidase inhibitor 12, PI-12, Serpin I1, SERPINI1, PI12
应用:WB,IHC,ICC,FCM
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The protein is primarily secreted by axons in the brain, and preferentially reacts with and inhibits tissue-type plasminogen activator. It is thought to play a role in the regulation of axonal growth and the development of synaptic plasticity. Mutations in this gene result in familial encephalopathy with neuroserpin inclusion bodies (FENIB), which is a dominantly inherited form of familial encephalopathy and epilepsy characterized by the accumulation of mutant neuroserpin polymers. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Description

This gene encodes a member of the serpin superfamily of
serine proteinase inhibitors. The protein is primarily secreted by
axons in the brain, and preferentially reacts with and inhibits
tissue-type plasminogen activator. It is thought to play a role in
the regulation of axonal growth and the development of synaptic
plasticity. Mutations in this gene result in familial
encephalopathy with neuroserpin inclusion bodies (FENIB), which is
a dominantly inherited form of familial encephalopathy and epilepsy
characterized by the accumulation of mutant neuroserpin polymers.
Multiple alternatively spliced variants, encoding the same protein,
have been identified.

Specification
Aliases	Neuroserpin, Peptidase inhibitor 12, PI-12, Serpin I1, SERPINI1, PI12
Entrez GeneID	5274
Swissprot	Q99574
WB Predicted band size	46.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This SERPINI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 19-45 amino acids from the N-terminal region of human SERPINI1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	Western blot analysis of lysate from SH-SY5Y cell line, using SERPINI1 Antibody (N-term)(Cat. #P30762). P30762 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Confocal immunofluorescent analysis of SERPINI1 Antibody (N-term)(Cat#P30762) with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	SERPINI1 Antibody (N-term) (Cat. #P30762) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	SERPINI1 antibody (N-term) (Cat. #P30762) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SERPINI1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.