库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to SFRP5

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别名:Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3
应用:WB,IHC
反应种属:Human, Mouse
规格:50μl/100μl

Description
Secreted frizzled-related protein 5 (SFRP5) is a member of
the SFRP family that contains a cysteine-rich domain homologous to
the putative Wnt-binding site of Frizzled proteins. SFRPs act as
soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be
involved in determining the polarity of photoreceptor cells in the
retina. SFRP5 is highly expressed in the retinal pigment
epithelium, and moderately expressed in the pancreas. [provided by
RefSeq].
Specification
Aliases Secreted frizzled-related protein 5, sFRP-5, Frizzled-related protein 1b, FRP-1b, Secreted apoptosis-related protein 3, SARP-3, SFRP5, FRP1B, SARP3
Entrez GeneID 6425
Swissprot Q5T4F7
WB Predicted band size 35.6kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This SFRP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 190-219 amino acids from the Central region of human SFRP5.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
IHC 1/500

All lanes : Anti-SFRP5 Antibody (Center) at 1:2000 dilution
Lane 1: Human liver lysate
Lane 2: Human pancreas lysate
Lane 3: Hela whole cell lysate
Lane 4: Mouse eyeball lysate
Lane 5: Mouse pancreas lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 36 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue using P34519 performed on the Leica® BOND RXm. Samples were incubated with primary antibody(1/500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。