中文 
别名:Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl
| Description |
|---|
| SIM1 and SIM2 genes are Drosophila single-minded (sim) gene homologs. SIM1 transcript was detected only in fetal kidney out of various adult and fetal tissues tested. Since the sim gene plays an important role in Drosophila development and has peak levels of expression during the period of neurogenesis,it was proposed that the human SIM gene is a candidate for involvement in certain dysmorphic features (particularly the facial and skull characteristics), abnormalities of brain development, and/or mental retardation of Down syndrome. |
| Specification | |
|---|---|
| Aliases | Single-minded homolog 1, Class E basic helix-loop-helix protein 14, bHLHe14, SIM1, BHLHE14 |
| Entrez GeneID | 6492 |
| Swissprot | P81133 |
| WB Predicted band size | 85.5kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SIM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human SIM1. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/2000 |
| IHC | 1/250 |
 |
Anti-SIM1 Antibody (N-term) at 1:2000 dilution + A549 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
 |
Immunohistochemical analysis of P34722 on paraffin-embedded Human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody. |
 |
Immunohistochemical analysis of P34722 on paraffin-embedded Human brain tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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