中文 
别名:Large neutral amino acids transporter small subunit 2, L-type amino acid transporter 2, hLAT2, Solute carrier family 7 member 8, SLC7A8, LAT2应用:WB,FCM
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| Sodium-independent, high-affinity transport of small and large neutral amino acids such as alanine, serine, threonine, cysteine, phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Acts as an amino acid exchanger. Has higher affinity for L-phenylalanine than LAT1 but lower affinity for glutamine and serine. L-alanine is transported at physiological concentrations. Plays a role in basolateral (re)absorption of neutral amino acids. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Plays an essential role in the reabsorption of neutral amino acids from the epithelial cells to the bloodstream in the kidney. |
| Specification | |
|---|---|
| Aliases | Large neutral amino acids transporter small subunit 2, L-type amino acid transporter 2, hLAT2, Solute carrier family 7 member 8, SLC7A8, LAT2 |
| Entrez GeneID | 23428 |
| Swissprot | Q9UHI5 |
| WB Predicted band size | 58.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SLC7A8 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 476-506 amino acids from human SLC7A8. |
| Application | |
|---|---|
| WB | 1/1000-1/2000 |
| FCM | 1/25 |
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All lanes : Anti-SLC7A8 Antibody (C-Term) at 1:1000-1:2000 dilution Lane 1: Human brain lysate Lane 2: Human kidney lysate Lane 3: Human liver lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 58 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Overlay histogram showing HepG2 cells stained with P34440(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34440, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1×10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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