Rabbit Polyclonal Antibody to Smad2 (Ab-220)

包装: 1G|100G|500G|1KG|100KG

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别名:JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.Riggins G.J., Nat. Genet. 13:347-349(1996).Zhang Y., Nature 383:168-172(1996).Eppert K., Cell 86:543-552(1996).

Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.Riggins G.J., Nat. Genet. 13:347-349(1996).Zhang Y., Nature 383:168-172(1996).Eppert K., Cell 86:543-552(1996).

Specification
Aliases	JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2
Entrez GeneID	4087;
Swissprot	Q15796
WB Predicted band size	60kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized non-phosphopeptide derived from human Smad2 around the phosphorylation site of threonine 220 (P-E-T(p)-P-P).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from RAW264.7 cells and 293 cells, using Smad2 (Ab-220) antibody P41668.
	Immunohistochemistry analysis of paraffin-embedded human brain tissue using Smad2 (Ab-220) antiobdy P41668.