Rabbit Polyclonal Antibody to Smad3 (Ab-204)

包装: 1G|100G|500G|1KG|100KG

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别名:JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
应用:WB,IHC
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.Zhang Y., Nature 383:168-172(1996).Riggins G.J., Nat. Genet. 13:347-349(1996).Arai T., Cancer Lett. 122:157-163(1998).

Description

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.Zhang Y., Nature 383:168-172(1996).Riggins G.J., Nat. Genet. 13:347-349(1996).Arai T., Cancer Lett. 122:157-163(1998).

Specification
Aliases	JV15-2; MAD-3; MADH3; Mad3; Mothers against DPP homolog 3
Entrez GeneID	4088;
Swissprot	P84022
WB Predicted band size	48kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Synthesized non-phosphopeptide derived from human Smad3 around the phosphorylation site of serine 204 (A-G-S(p)-P-N).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from HUVEC cells, using Smad3 (Ab-204) antibody P41669.
	Immunohistochemistry analysis of paraffin-embedded human colon colon tissue using Smad3 (Ab-204) antibody P41669.