Rabbit Polyclonal Antibody to SMAD4

包装: 1G|100G|500G|1KG|100KG

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别名:JIP, DPC4, MADH4, MYHRS
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Description

This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.

Specification
Aliases	JIP, DPC4, MADH4, MYHRS
Swissprot	Q13485
WB Predicted band size	60 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	Fusion protein of human SMAD4
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/500
IHC	1/50-1/200
ELISA	1/1000-1/2000

	Gel: 10%SDS-PAGE, Lysate: 30 μg, Lane 1-2: Human liver cancer tissue, Hela cells, Primary antibody: P07024(SMAD4 Antibody) at dilution 1/150, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
	The image is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using P07024(SMAD4 Antibody) at dilution 1/40. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P07024(SMAD4 Antibody) at dilution 1/40. (Original magnification: ×200)