库存-现货
瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to SNRPD3 (N-term)

现货 支持定制

  • 满200元免运费
  • 正品保障 提供发票
  • 现货闪电 当时发货

别名:Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3, SNRPD3
应用:WB,IHC
反应种属:Human, Mouse
规格:50μl/100μl

Description
The protein encoded by this gene belongs to the small
nuclear ribonucleoprotein core protein family. It is required for
pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis.
Specification
Aliases Small nuclear ribonucleoprotein Sm D3, Sm-D3, snRNP core protein D3, SNRPD3
Entrez GeneID 6634
Swissprot P62318
WB Predicted band size 13.9kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This SNRPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human SNRPD3.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000-1/4000
IHC 1/500

Anti-SNRPD3 Antibody (N-term) at 1:2000 dilution + COLO 205 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-SNRPD3 Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: MCF-7 whole cell lysate
Lane 3: 293 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: HL-60 whole cell lysate
Lane 6: Mouse heart tissue lysate
Lane 7: NIH/3T3 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 14 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human colon tissue using P34687 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34687 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

暂无评论

发表回复

您的邮箱地址不会被公开。 必填项已用 * 标注


本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。