Rabbit Polyclonal Antibody to SPAG4

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别名:SUN4; CT127
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.

Description

The mammalian sperm flagellum contains two cytoskeletal structures associated with the axoneme: the outer dense fibers surrounding the axoneme in the midpiece and principal piece and the fibrous sheath surrounding the outer dense fibers in the principal piece of the tail. Defects in these structures are associated with abnormal tail morphology, reduced sperm motility, and infertility. In the rat, the protein encoded by this gene associates with an outer dense fiber protein via a leucine zipper motif and localizes to the microtubules of the manchette and axoneme during sperm tail development.

Specification
Aliases	SUN4; CT127
Swissprot	Q9NPE6
WB Predicted band size	48 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human SPAG4
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/100-1/300
ELISA	1/2000-1/5000

	Gel: 8%SDS-PAGE, Lysate: 40 μg, Lane: NIH/3T3 cell lysate, Primary antibody: P04354(SPAG4 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
	The image is immunohistochemistry of paraffin-embedded Human colon cancer tissue using P04354(SPAG4 Antibody) at dilution 1/70. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human lung cancer tissue using P04354(SPAG4 Antibody) at dilution 1/70. (Original magnification: ×200)