Rabbit Polyclonal Antibody to STAM

包装: 1G|100G|500G|1KG|100KG

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别名:Signal transducing adapter molecule 1, STAM-1, STAM, STAM1
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). STAM for ‘signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540-amino acid protein precipitated by anti-phosphotyrosine. Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.

Description

Stimulation of cells with cytokines initiates a signal transduction cascade involving cytokine receptors, Janus kinases (JAKs) and signal transducers and activators of transcription (STATs). STAM for ‘signal-transducing adaptor molecule, induced after stimulation of cells with cytokine IL2, is a component of signal transduction downstream of JAK3.1 Human STAM cDNA cloned from a T-cell cDNA library encodes a 540-amino acid protein precipitated by anti-phosphotyrosine. Northern blot analysis indicates that STAM is expressed as a 2.9-kb message in a wide variety of tissue and cell types. The STAM sequence contains a Src-homology 3 (SH3) domain and an immunoreceptor tyrosine-based activation motif (ITAM). It has been suggested that STAM acts as an adaptor molecule in signal transduction pathways from cytokine receptors.

Specification
Aliases	Signal transducing adapter molecule 1, STAM-1, STAM, STAM1
Entrez GeneID	8027
Swissprot	Q92783
WB Predicted band size	59.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This STAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-356 amino acids from the C-terminal region of human STAM.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Application
WB	1/1000
IHC	1/100-1/500

	Western blot analysis of anti-STAM Antibody (C-term) (Cat.#P30072) in Hela cell line lysates (35ug/lane). STAM (arrow) was detected using the purified Pab.
	Western blot analysis of STAM (arrow) using rabbit polyclonal STAM Antibody (P341) (Cat.#P30072). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the STAM gene.
	Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.