中文 
别名:Brachyury protein, Protein T, T应用:WB,ICC
反应种属:Human, Mouse
规格：50μl/100μl
| Description |
|---|
| The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells. |
| Specification | |
|---|---|
| Aliases | Brachyury protein, Protein T, T |
| Entrez GeneID | 6862 |
| Swissprot | O15178 |
| WB Predicted band size | 47.4kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This T antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 15-43 amino acids from the N-terminal region of human T. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/2000 |
| ICC | 1/25 |
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Anti-T Antibody (N-term) at 1:2000 dilution + NCI-H460 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 47 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling TBXT with P34475 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus, cytoplasm weakly staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/500 dilution (red). The nuclear counter stain is DAPI (blue). |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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