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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to TBX6

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别名:T-box transcription factor TBX6, T-box protein 6, TBX6
应用:WB,ICC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
This gene is a member of a phylogenetically conserved
family of genes that share a common DNA-binding domain, the T-box.
T-box genes encode transcription factors involved in the regulation
of developmental processes. Knockout studies in mice indicate that
this gene is important for specification of paraxial mesoderm
structures.
Specification
Aliases T-box transcription factor TBX6, T-box protein 6, TBX6
Entrez GeneID 6911
Swissprot O95947
WB Predicted band size 47.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This TBX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 264-293 amino acids from the Central region of human TBX6.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/500
ICC 1/10-1/50

Anti-TBX6 Antibody (Center) at 1:500 dilution + Mouse spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 47 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent confocal image of Hela cell stained with TBX6 Antibody (Center)(Cat#P34707). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TBX6 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TBX6 immunoreactivity is localized to nucleus significantly.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。