Rabbit Polyclonal Antibody to TCF3

包装: 1G|100G|500G|1KG|100KG

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别名:Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1
应用:WB,IHC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
The TCF3 gene, also called E2A, encodes 2 basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. E12 and E47 are involved in regulation of immunoglobulin gene expression (Bain et al., 1994 [PubMed 8001125]).

Specification
Aliases	Transcription factor E2-alpha, Class B basic helix-loop-helix protein 21, bHLHb21, Immunoglobulin enhancer-binding factor E12/E47, Immunoglobulin transcription factor 1, Kappa-E2-binding factor, Transcription factor 3, TCF-3, Transcription factor ITF-1, TCF3, BHLHB21, E2A, ITF1
Entrez GeneID	6929
Swissprot	P15923
WB Predicted band size	67.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This TCF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506-534 amino acids from the C-terminal region of human TCF3.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
FCM	1/10-1/50

	All lanes : Anti-TCF3 Antibody (C-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: Ramos whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	TCF3 Antibody (C-term) (Cat. #P33614) western blot analysis in human uterine tumor tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).
	TCF3 Antibody (C-term) (Cat. #P33614) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the TCF3 antibody detected the TCF3 protein (arrow).
	TCF3 Antibody (C-term) (Cat. #P33614) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	TCF3 Antibody (C-term) (Cat. #P33614)immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TCF3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.