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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to TEF

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别名:Thyrotroph embryonic factor, TEF, KIAA1655
应用:WB
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
Thyrotroph embryonic factor (TEF), a transcription factor,
is a member of the PAR (proline and acidic amino acid-rich)
subfamily of basic region/leucine zipper (bZIP) transcription
factors. It is expressed in a broad range of cells and tissues in
adult animals, however, during embryonic development, TEF
expression appears to be restricted to the developing anterior
pituitary gland, coincident with the appearance of
thyroid-stimulating hormone, beta (TSHB). Indeed, TEF can bind to,
and transactivate the TSHB promoter. It shows homology (in the
functional domains) with other members of the PAR-bZIP subfamily of
transcription factors, which include albumin D box-binding protein
(DBP), human hepatic leukemia factor (HLF) and chicken vitellogenin
gene-binding protein (VBP); VBP is considered the chicken homologue
of TEF. Different members of the subfamily can readily form
heterodimers, and share DNA-binding, and transcriptional regulatory
properties. Alternatively spliced transcript variants encoding
different isoforms have been found for this gene. [provided by
RefSeq].
Specification
Aliases Thyrotroph embryonic factor, TEF, KIAA1655
Entrez GeneID 7008
Swissprot Q10587
WB Predicted band size 33.2kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This TEF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 213-240 amino acids from the C-terminal region of human TEF.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000-1/2000

Anti-TEF Antibody (C-term) at 1:2000 dilution + human cerebellum lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 33 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

TEF Antibody (C-term) (Cat. #P34012) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the TEF antibody detected the TEF protein (arrow).

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。