Rabbit Polyclonal Antibody to TK (Ab-13)

包装: 1G|100G|500G|1KG|100KG

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别名:EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell.TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division. Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive. These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response.N Singh, Mol. Cell. Biol., May 1991; 11: 2362 – 2374. Bin Tian, Am. J. Pathol., Aug 2003; 163: 789. Ren篓娄 Gerolami, Cancer Res., Feb 2000; 60: 993 – 1001. Catia Traversari, Blood, Jun 2007; 109: 4708 – 4715

Description

Thymidine kinases play a critical role in generating the DNA synthetic precursor deoxythymidine triphosphate (dTTP) by catalyzing the phosphotransfer of phosphate from ATP to deoxythymidine (dT) and thymidine (T) in the cell.TK1 expression and activity is regulated in a cell cycle-dependent manner, accumulating during G1-phase to peak levels in S-phase before being degraded prior to cell division. Stability, but not activity, may be regulated via phosphorylation of TK1 at Ser13 by Cdc2 and/or Cdk2, but the precise mode of regulation remains elusive. These observations indicate that TK1 might be a useful marker of cell proliferation; however, recent studies have shown that TK1 plays a more significant role in the DNA damage response.N Singh, Mol. Cell. Biol., May 1991; 11: 2362 – 2374. Bin Tian, Am. J. Pathol., Aug 2003; 163: 789. Ren篓娄 Gerolami, Cancer Res., Feb 2000; 60: 993 – 1001. Catia Traversari, Blood, Jun 2007; 109: 4708 – 4715

Specification
Aliases	EC 2.7.1.21; KITH; TK-1; TK1; Thymidine kinase
Entrez GeneID	7083;
Swissprot	P04183
WB Predicted band size	25kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	Synthesized non-phosphopeptide derived from human TK around the phosphorylation site of serine 13 (P-G-S(p)-P-S).
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100
ICC	1/100-1/500

	Western blot analysis of extracts from COLO205 cells, using TK (Ab-13) antibody P41653.
	Immunofluorescence analysis of HepG2 cells, using TK (Ab-13) antibody P41653.
	Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using TK (Ab-13) antibody P41653.