Rabbit Polyclonal Antibody to TOP3B

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别名:DNA topoisomerase 3-beta-1; EC 5.99.1.2; DNA topoisomerase III beta-1; TOP3B; TOP3B1
应用:WB
反应种属:Human,Mouse
规格：50μl/100μl

Description
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3′-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3′-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.Ng S.-W., Nucleic Acids Res. 27:993-1000(1999).Kawasaki K., Genome Res. 7:250-261(1997).Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).

Description

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3′-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3′-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.Ng S.-W., Nucleic Acids Res. 27:993-1000(1999).Kawasaki K., Genome Res. 7:250-261(1997).Collins J.E., Genome Biol. 5:RESEARCH84.1-RESEARCH84.11(2004).

Specification
Aliases	DNA topoisomerase 3-beta-1; EC 5.99.1.2; DNA topoisomerase III beta-1; TOP3B; TOP3B1
Entrez GeneID	8940;
Swissprot	O95985
WB Predicted band size	96kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized peptide derived from internal of human TOP3B.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.