Rabbit Polyclonal Antibody to Topoisomerase I

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别名:TOP1; DNA topoisomerase 1; DNA topoisomerase I
应用:WB,IHC,ICC,FCM
反应种属:Human,Mouse
规格：50μl/100μl

Description
Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand.

Description

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3′-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5′-OH DNA strand.

Specification
Aliases	TOP1; DNA topoisomerase 1; DNA topoisomerase I
Entrez GeneID	7150
Swissprot	P11387
WB Predicted band size	Calculated MW: 91 kDa; Observed MW: 91 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	A synthesized peptide derived from human TOP1
Formulation	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200
FCM	1/50-1/100

	Western blot analysis of TOP1 in Jurkat lysates using Topoisomerase I antibody.
	Immunohistochemistry analysis of paraffin-embedded Human colon using TOP1 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.