Rabbit Polyclonal Antibody to TriMethyl-Histone H3 (Lys14)

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别名:H3K14me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
应用:WB,IHC,ICC,IP
反应种属:Human,Mouse,Rat
规格：50μl/100μl

Description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Specification
Aliases	H3K14me3; H3 histone; HIST1H3A; Histone cluster 1; H3a
Entrez GeneID	8290
Swissprot	Q16695
WB Predicted band size	Calculated MW: 16 kDa; Observed MW: 16 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	A synthetic peptide of human TriMethyl-Histone H3-K14
Formulation	Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Application
IP	1/20
WB	1/500-1/1000
IHC	1/50-1/100
ICC	1/50-1/200

	Western blot analysis of TriMethyl-Histone H3 (Lys14) in various cell lines lysates using TriMethyl-Histone H3K14 antibody.
	Immunofluorescence analysis of TriMethyl-Histone H3 (Lys14) in C6 using TriMethyl-Histone H3K14 antibody,and DAPI (blue).