中文 
别名:tRNA-splicing endonuclease subunit Sen54; tRNA-intron endonuclease Sen54; HsSen54; TSEN54; SEN54应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl
| Description |
|---|
| Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5′ and 3′ splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2′,3′ cyclic phosphate and 5′-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3′-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3′-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events.Zody M.C., Nature 440:1045-1049(2006).Paushkin S.V., Cell 117:311-321(2004).Ota T., Nat. Genet. 36:40-45(2004). |
| Specification | |
|---|---|
| Aliases | tRNA-splicing endonuclease subunit Sen54; tRNA-intron endonuclease Sen54; HsSen54; TSEN54; SEN54 |
| Entrez GeneID | 283989; |
| Swissprot | Q7Z6J9 |
| WB Predicted band size | 59kDa |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from internal of human TSEN54. |
| Formulation | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Application | |
|---|---|
| WB | 1/500-1/3000 |
| IHC | 1/50-1/100 |
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Western blot analysis of extracts from 3T3 cells and Jurkat cells, using TSEN54 antibody P42645. |
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Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using TSEN54 antibody P42645. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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