别名:Tubulin beta-2B chain, TUBB2B应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl
Description |
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The protein encoded by this gene is a beta isoform of tubulin, which binds GTP and is a major component of microtubules. This gene is highly similar to TUBB2A and TUBB2C. Defects in this gene are a cause of asymmetric polymicrogyria. [provided by RefSeq]. |
Specification | |
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Aliases | Tubulin beta-2B chain, TUBB2B |
Entrez GeneID | 347733 |
Swissprot | Q9BVA1 |
WB Predicted band size | 50.0kDa |
Host/Isotype | Rabbit IgG |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B. |
Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Application | |
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WB | 1/2000 |
IHC | 1/2000 |
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All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: MDA-MB-231 whole cell lysate Lane 4: K562 whole cell lysate Lane 5: Mouse brain tissue lysate Lane 6: Mouse cerebellum tissue lysate Lane 7: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
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Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
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Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。
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