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Rabbit Polyclonal Antibody to TUBB2B (N-term)

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别名:Tubulin beta-2B chain, TUBB2B
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
The protein encoded by this gene is a beta isoform of
tubulin, which binds GTP and is a major component of microtubules.
This gene is highly similar to TUBB2A and TUBB2C. Defects in this
gene are a cause of asymmetric polymicrogyria. [provided by
RefSeq].
Specification
Aliases Tubulin beta-2B chain, TUBB2B
Entrez GeneID 347733
Swissprot Q9BVA1
WB Predicted band size 50.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This TUBB2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-39 amino acids from the N-terminal region of human TUBB2B.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/2000
IHC 1/2000

All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti-TUBB2B Antibody (N-term) at 1:4000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: MDA-MB-231 whole cell lysate
Lane 4: K562 whole cell lysate
Lane 5: Mouse brain tissue lysate
Lane 6: Mouse cerebellum tissue lysate
Lane 7: PC-12 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded Human brain tissue using P34685 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:2000) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。