Rabbit Polyclonal Antibody to TUT1

包装: 1G|100G|500G|1KG|100KG

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别名:U6 snRNA-specific terminal uridylyltransferase 1; EC 2.7.7.52; U6-TUTase; RNA-binding protein 21; RNA-binding motif protein 21
应用:WB,IHC
反应种属:Human,Mouse
规格：50μl/100μl

Description
Poly(A) polymerase that creates the 3′-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3′-end cleavage of pre-mRNAs: binds to the 3’UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3’UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3′-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.Ota T., Nat. Genet. 36:40-45(2004).The MGC Project Team, Genome Res. 14:2121-2127(2004).Trippe R.,RNA 12:1494-1504(2006).

Description

Poly(A) polymerase that creates the 3′-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3′-end cleavage of pre-mRNAs: binds to the 3’UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3’UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3′-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.Ota T., Nat. Genet. 36:40-45(2004).The MGC Project Team, Genome Res. 14:2121-2127(2004).Trippe R.,RNA 12:1494-1504(2006).

Specification
Aliases	U6 snRNA-specific terminal uridylyltransferase 1; EC 2.7.7.52; U6-TUTase; RNA-binding protein 21; RNA-binding motif protein 21
Entrez GeneID	64852;
Swissprot	Q9H6E5
WB Predicted band size	95kDa
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse
Immunogen	Synthesized peptide derived from internal of human TUT1.
Formulation	Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Application
WB	1/500-1/3000
IHC	1/50-1/100

	Western blot analysis of extracts from 3T3 cells, using TUT1 antiobdy P42649.
	Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using TUT1 antibody P42649.