中文 
别名:Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499)应用:WB,IHC
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| Ubiquitination requires a ubiquitin-activating enzyme, a ubiquitin-conjugating enzyme, and usually a substrate-specific ubiquitin-protein ligase. Multiubiquitination needs a fourth factor, to bind to the ubiquitin moieties of preformed conjugates and catalyze ubiquitin chain assembly in conjunction with the first three proteins.1 Human protein KIAA0126, with a predicted 1,073 amino acid residues, is a member of the novel protein family defined by ubiquitination conjugation factor E4.2 In yeast, E4 activity is linked to cell survival under stress conditions, indicating that eucaryotes use E4-dependent proteolysis pathways for multiple cellular functions. |
| Specification | |
|---|---|
| Aliases | Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499) |
| Entrez GeneID | 9354 |
| Swissprot | Q14139 |
| WB Predicted band size | 122.6kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1037-1067 amino acids from the C-terminal region of human UBE4A. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
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Anti-UBE4A Antibody (T1052) at 1:1000 dilution + K562 whole cell lysate
Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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Western blot analysis of anti-UBE4A Antibody (C-term) (Cat.#P33626) in NCI-H460 and CEM cell line lysates (35ug/lane). UBE4A (arrow) was detected using the purified Pab. |
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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