中文 
别名:Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499)应用:WB,IHC
反应种属:Human
规格：50μl/100μl
| Description |
|---|
| The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. |
| Specification | |
|---|---|
| Aliases | Ubiquitin conjugation factor E4 A, 632-, UBE4A (HGNC:12499) |
| Entrez GeneID | 9354 |
| Swissprot | Q14139 |
| WB Predicted band size | 122.6kDa |
| Host/Isotype | Rabbit IgG |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This UBE4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 451-479 amino acids from the Central region of human UBE4A. |
| Formulation | Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application | |
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
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All lanes : Anti-UBE4A Antibody (Center) at 1:1000 dilution Lane 1: Caco2 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Predicted band size : 123 kDa Blocking/Dilution buffer: 5% NFDM/TBST. |
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UBE4A Antibody (Center) (Cat. #P33989) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the UBE4A antibody detected the UBE4A protein (arrow). |
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UBE4A Antibody (Center) (P33989)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UBE4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. |
本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。
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