Rabbit Polyclonal Antibody to UGT1A1 (N-term)

包装: 1G|100G|500G|1KG|100KG

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别名:UDP-glucuronosyltransferase 1-1, UDPGT 1-1, UGT1*1, UGT1-01, UGT11, Bilirubin-specific UDPGT isozyme 1, hUG-BR1, UDP-glucuronosyltransferase 1-A, UGT-1A, UGT1A, UDP-glucuronosyltransferase 1A1, UGT1A1, GNT1, UGT1
应用:WB,IHC,ICC
反应种属:Human
规格：50μl/100μl

Description
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5′ exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

Description

This gene encodes a UDP-glucuronosyltransferase, an enzyme
of the glucuronidation pathway that transforms small lipophilic
molecules, such as steroids, bilirubin, hormones, and drugs, into
water-soluble, excretable metabolites. This gene is part of a
complex locus that encodes several UDP-glucuronosyltransferases.
The locus includes thirteen unique alternate first exons followed
by four common exons. Four of the alternate first exons are
considered pseudogenes. Each of the remaining nine 5′ exons may be
spliced to the four common exons, resulting in nine proteins with
different N-termini and identical C-termini. Each first exon
encodes the substrate binding site, and is regulated by its own
promoter. The preferred substrate of this enzyme is bilirubin,
although it also has moderate activity with simple phenols,
flavones, and C18 steroids. Mutations in this gene result in
Crigler-Najjar syndromes types I and II and in Gilbert syndrome.

Specification
Aliases	UDP-glucuronosyltransferase 1-1, UDPGT 1-1, UGT1*1, UGT1-01, UGT11, Bilirubin-specific UDPGT isozyme 1, hUG-BR1, UDP-glucuronosyltransferase 1-A, UGT-1A, UGT1A, UDP-glucuronosyltransferase 1A1, UGT1A1, GNT1, UGT1
Entrez GeneID	54658
Swissprot	P22309
WB Predicted band size	59.6kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This UGT1A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 65-90 amino acids from the N-terminal region of human UGT1A1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50

	UGT1A1 Antibody (N-term) (Cat. #P31373) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the UGT1A1 antibody detected the UGT1A1 protein (arrow).
	Confocal immunofluorescent analysis of UGT1A1 Antibody (N-term)(Cat#P31373) with HepG2 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	UGT1A1 Antibody (N-term) (Cat. #P31373)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of UGT1A1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.