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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to ULK2 (Center S323)

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别名:Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2, ULK2, KIAA0623
应用:WB,ICC
反应种属:Human
规格:50μl/100μl

Description
This gene encodes a protein that is similar to a
serine/threonine kinase in C. elegans which is involved in axonal
elongation. The structure of this protein is similar to the C.
elegans protein in that both proteins have an N-terminal kinase
domain, a central proline/serine rich (PS) domain, and a C-terminal
(C) domain. The gene is located within the Smith-Magenis syndrome
region on chromosome 17. Alternatively spliced transcript variants
encoding the same protein have been identified. [provided by
RefSeq].
Specification
Aliases Serine/threonine-protein kinase ULK2, Unc-51-like kinase 2, ULK2, KIAA0623
Entrez GeneID 9706
Swissprot Q8IYT8
WB Predicted band size 112.7kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human
Immunogen This ULK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 300-327 amino acids from the Central region of human ULK2.
Formulation Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Application
WB 1/1000
ICC 1/10-1/50

ULK2 Antibody (S323) (Cat. #P32178) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the ULK2 antibody detected the ULK2 protein (arrow).
Fluorescent image of U251 cell stained with ULK2 Antibody (Center S323)(Cat#P32178).U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ULK2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).ULK2 immunoreactivity is localized to Nucleus significantly.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。