Rabbit Polyclonal Antibody to UPF1

包装: 1G|100G|500G|1KG|100KG

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别名:HUPF1; NORF1; RENT1; smg-2; pNORF1
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.

Description

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Alternative splicing results in multiple transcript variants.

Specification
Aliases	HUPF1; NORF1; RENT1; smg-2; pNORF1
Swissprot	Q92900
WB Predicted band size	124 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human UPF1
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/200-1/1000
IHC	1/50-1/200
ELISA	1/1000-1/5000

	Gel: 6%SDS-PAGE, Lysate: 60 μg, Lane: Raji cells, Primary antibody: P04601(UPF1 Antibody) at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 minutes
	The image is immunohistochemistry of paraffin-embedded Human liver cancer tissue using P04601(UPF1 Antibody) at dilution 1/35. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P04601(UPF1 Antibody) at dilution 1/35. (Original magnification: ×200)