Rabbit Polyclonal Antibody to USP29

包装: 1G|100G|500G|1KG|100KG

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别名:Ubiquitin carboxyl-terminal hydrolase 29, Deubiquitinating enzyme 29, Ubiquitin thioesterase 29, Ubiquitin-specific-processing protease 29, USP29
应用:WB
反应种属:Human
规格：50μl/100μl

Description
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Description

Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26 S proteasome are coupled to multiple copies of ubiquitin. However, attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity. An additional level of ubiquitin regulation, deubiquitination, is catalyzed by proteases called deubiquitinating enzymes, which fall into four distinct families. Ubiquitin C-terminal hydrolases, ubiquitin-specific processing proteases (USPs),1 OTU-domain ubiquitin-aldehyde-binding proteins, and Jab1/Pad1/MPN-domain-containing metallo-enzymes. Among these four families, USPs represent the most widespread and represented deubiquitinating enzymes across evolution. USPs tend to release ubiquitin from a conjugated protein. They display similar catalytic domains containing conserved Cys and His boxes but divergent N-terminal and occasionally C-terminal extensions, which are thought to function in substrate recognition, subcellular localization, and protein-protein interactions.

Specification
Aliases	Ubiquitin carboxyl-terminal hydrolase 29, Deubiquitinating enzyme 29, Ubiquitin thioesterase 29, Ubiquitin-specific-processing protease 29, USP29
Entrez GeneID	57663
Swissprot	Q9HBJ7
WB Predicted band size	104.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This USP29 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 660-690 amino acids from the Central region of human USP29.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/2000

All lanes : Anti-USP29 Antibody (Center) at 1:2000 dilution
Lane 1: 293 cell lysate
Lane 2: HACAT cell lysate
Lane 3: THP-1 cell lysate
Lane 4: T47D cell lysate
Lane 5: CCRF-CEM cell lysate
Lane 6: PC-3 cell lysate

Lysates/proteins at 20 µg per lane.

Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution.

Observed band size : 95 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的，不可用于人类或动物的临床诊断或治疗，非药用，非食用。

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