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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to VAC14 (N-Term)

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别名:Protein VAC14 homolog, Tax1-binding protein 2, VAC14, TAX1BP2, TRX
应用:WB,IHC
反应种属:Human, Mouse, Rat
规格:50μl/100μl

Description
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Specification
Aliases Protein VAC14 homolog, Tax1-binding protein 2, VAC14, TAX1BP2, TRX
Entrez GeneID 55697
Swissprot Q08AM6
WB Predicted band size 88.0kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse, Rat
Immunogen This VAC14 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 125-159 amino acids from human VAC14.
Application
WB 1/2000
IHC 1/100-1/500

Anti-VAC14 Antibody (N-Term) at 1:2000 dilution + human spleen lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 88 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

P33411 staining VAC14 in human kidney tissue sections by Immunohistochemistry (IHC-P – paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。