Rabbit Polyclonal Antibody to Vimentin

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别名:Vimentin, VIM
应用:WB,ICC
反应种属:Human, Mouse
规格：50μl/100μl

Description
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.

Description

This gene encodes a member of the intermediate filament
family. Intermediate filamentents, along with microtubules and
actin microfilaments, make up the cytoskeleton. The protein encoded
by this gene is responsible for maintaining cell shape, integrity
of the cytoplasm, and stabilizing cytoskeletal interactions. It is
also involved in the immune response, and controls the transport of
low-density lipoprotein (LDL)-derived cholesterol from a lysosome
to the site of esterification. It functions as an organizer of a
number of critical proteins involved in attachment, migration, and
cell signaling. Mutations in this gene causes a dominant,
pulverulent cataract.

Specification
Aliases	Vimentin, VIM
Entrez GeneID	7431
Swissprot	P08670
WB Predicted band size	53.7kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/2000
ICC	1/50

	All lanes : Anti-VIME Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
	Vimentin Antibody flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).Alexa Fluor 488-conjugated donkey anti-rabbit lgG secondary antibodies were used for the analysis.