Rabbit Polyclonal Antibody to VPS35

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别名:MEM3; PARK17
应用:WB,IHC
反应种属:Human, Mouse
规格：50μl/100μl

Description
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Description

This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex.

Specification
Aliases	MEM3; PARK17
Swissprot	Q96QK1
WB Predicted band size	92 kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse
Immunogen	Synthetic peptide of human VPS35
Formulation	pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Application
WB	1/500-1/2000
IHC	1/20-1/100
ELISA	1/5000-1/10000

	Gel: 6%SDS-PAGE, Lysate: 40 μg, Lane 1-7: Hela, HEPG2, Raji, NIH/3T3 and A549 cell lysates, Human cerebella tissue and Mouse brain tissue lysates, Primary antibody: P12900(VPS35 Antibody) at dilution 1/250, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
	The image is immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using P12900(VPS35 Antibody) at dilution 1/25. (Original magnification: ×200)
	The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using P12900(VPS35 Antibody) at dilution 1/25. (Original magnification: ×200)