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瑞威尔生物科技 REVERE

Rabbit Polyclonal Antibody to WDR82 (N-term)

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别名:WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A
应用:WB,IHC
反应种属:Human, Mouse
规格:50μl/100μl

Description
Regulatory component of the SET1 complex implicated in the tethering of this complex to transcriptional start sites of active genes. Facilitates histone H3 ‘Lys-4’ methylation via recruitment of the SETD1A or SETD1B to the ‘Ser-5’ phosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A). Component of PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Specification
Aliases WD repeat-containing protein 82, Protein TMEM113, Swd2, WDR82, TMEM113, WDR82A
Entrez GeneID 80335
Swissprot Q6UXN9
WB Predicted band size 35.1kDa
Host/Isotype Rabbit IgG
Storage Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity Human, Mouse
Immunogen This WDR82 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 12~46 amino acids from the N-terminal region of human WDR82.
Application
WB 1/1000
IHC 1/500

All lanes : Anti-WDR82 Antibody (N-term) at 1:1000 dilution
Lane 1: NIH/3T3 whole cell lysate
Lane 2: NCCIT whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: HCT116 whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.

Predicted band size : 35 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human placenta tissue using P34608 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
Immunohistochemical analysis of paraffin-embedded human brain tissue using P34608 performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。

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本公司的所有产品仅用于科学研究或者工业应用等非医疗目的,不可用于人类或动物的临床诊断或治疗,非药用,非食用。