Rabbit Polyclonal Antibody to WT1 (Center E361)

包装: 1G|100G|500G|1KG|100KG

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别名:Wilms tumor protein, WT33, WT1
应用:WB,IHC,ICC,FCM
反应种属:Human, Mouse, Rat
规格：50μl/100μl

Description
This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm’s tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

Description

This gene encodes a transcription factor that contains
four zinc-finger motifs at the C-terminus and a
proline/glutamine-rich DNA-binding domain at the N-terminus. It has
an essential role in the normal development of the urogenital
system, and it is mutated in a small subset of patients with Wilm’s
tumors. This gene exhibits complex tissue-specific and polymorphic
imprinting pattern, with biallelic, and monoallelic expression from
the maternal and paternal alleles in different tissues. Multiple
transcript variants have been described. In several variants, there
is evidence for the use of a non-AUG (CUG) translation initiation
site upstream of and in-frame with the first AUG. Authors of
PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA
editing in human and rat, and that this process is
tissue-restricted and developmentally regulated. [provided by
RefSeq].

Specification
Aliases	Wilms tumor protein, WT33, WT1
Entrez GeneID	7490
Swissprot	P19544
WB Predicted band size	49.2kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human, Mouse, Rat
Immunogen	This WT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 346-375 amino acids from the Central region of human WT1.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500
ICC	1/10-1/50
FCM	1/10-1/50

	All lanes : Anti-WT1 Antibody (Center E361) at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: Raji whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: Mouse testis lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 49 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
	Confocal immunofluorescent analysis of WT1 Antibody (Center E361)(Cat. #P34639) with MCF-7 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
	WT1 Antibody (Center E361) (Cat. #P34639) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	WT1 Antibody (Center E361) (Cat. #P34639)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of WT1 Antibody (Center E361) for immunohistochemistry. Clinical relevance has not been evaluated.