Rabbit Polyclonal Antibody to ZP4

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别名:Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
应用:WB,IHC
反应种属:Human
规格：50μl/100μl

Description
The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4.

Description

The zona pellucida is an extracellular matrix that
surrounds the oocyte and early embryo. It is composed primarily of
three or four glycoproteins with various functions during
fertilization and preimplantation development. The nascent protein
contains a N-terminal signal peptide sequence, a conserved ZP
domain, a consensus furin cleavage site, and a C-terminal
transmembrane domain. It is hypothesized that furin cleavage
results in release of the mature protein from the plasma membrane
for subsequent incorporation into the zona pellucida matrix.
However, the requirement for furin cleavage in this process remains
controversial based on mouse studies. Previously, this gene has
been referred to as ZP1 or ZPB and thought to have similar
functions as mouse Zp1. However, a human gene with higher
similarity and chromosomal synteny to mouse Zp1 has been assigned
the symbol ZP1 and this gene has been assigned the symbol ZP4.

Specification
Aliases	Zona pellucida sperm-binding protein 4, Zona pellucida glycoprotein 4, Zp-4, Zona pellucida protein B, Processed zona pellucida sperm-binding protein 4, ZP4, ZPB
Entrez GeneID	57829
Swissprot	Q12836
WB Predicted band size	59.4kDa
Host/Isotype	Rabbit IgG
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This ZP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 445-474 amino acids from the C-terminal region of human ZP4.
Formulation	Purified polyclonal antibody supplied in PBS with 0.05% sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.

Application
WB	1/1000
IHC	1/100-1/500

	ZP4 Antibody (C-term) (Cat. #P31138) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the ZP4 antibody detected the ZP4 protein (arrow).
	ZP4 Antibdy (C-term) (Cat. #P31138)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ZP4 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.